Identification of Uric Acid Crystals Accumulation in Human and Animal Tissues Using Combined Morphological and Raman Spectroscopy Analysis
Teodoru Soare,
Ana Maria Iordache,
George Nicolae,
Stefan-Marian Iordache,
Cosmin Baciu,
Silviu Marinescu,
Raluca Ioana Rizac,
Manuella Militaru
Affiliations
Teodoru Soare
Department of Pathology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Splaiul Independentei Street, No. 105, Sector 5, 050097 Bucharest, Romania
Ana Maria Iordache
Optospintronics Department, National Institute for Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
George Nicolae
Department of Pathology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Splaiul Independentei Street, No. 105, Sector 5, 050097 Bucharest, Romania
Stefan-Marian Iordache
Optospintronics Department, National Institute for Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, Romania
Cosmin Baciu
Department 14 Orthopedy-Traumatology-ATI, University of Medicine and Pharmacy Carol Davilla (UMFCD), Dionisie Lupu Street, No. 37, Sector 2, 020021 Bucharest, Romania
Silviu Marinescu
Department 11-Plastic and Reconstructive Surgery, Pediatric Surgery, University of Medicine and Pharmacy Carol Davilla (UMFCD), Eroii Sanitari Bvd., No. 8, Sector 5, 050471 Bucharest, Romania
Raluca Ioana Rizac
Department of Pathology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Splaiul Independentei Street, No. 105, Sector 5, 050097 Bucharest, Romania
Manuella Militaru
Department of Pathology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Splaiul Independentei Street, No. 105, Sector 5, 050097 Bucharest, Romania
Gout is a metabolic condition, common to animals and humans, issuing from the excessive accumulation of end products of proteins degradation. In this study, histopathological and cytological examinations, combined with Raman spectroscopy, have been performed to investigate tissue samples from reptiles, chickens, and humans, presenting lesions produced by uric acid accumulation. As a result of classic processing and staining techniques commonly used in the anatomopathological diagnosis, uric acid crystals lose their structural characteristics, thus making difficult a precise diagnostic. Therefore, complementary diagnostic methods, such as Raman spectroscopy, are needed. This study compares from several perspectives the above mentioned diagnostic methods, concluding that Raman spectroscopy provides highlights in the diagnosis of gout in humans and animals, also adding useful information to differential diagnosis of lesions.
