The interactive effect of force swimming on the amount of SOD & CAT Cardiomyocyte in male rats poisoned Hydrogen peroxide

Abstract

Introduction The heart muscle, as a continuous oxidative tissue, is one of the most susceptible tissues for oxidative damage caused by reactive oxygen species. SOD and CAT enzymes play an important role in reducing oxidative stress.The aim of this study is to determine the effect of 6-weeks duration force swimming on the levels of SOD and CAT cardiomyocyte in rats poisoned by hydrogen peroxide. Materials and Methods In this study included 32 Wistar male rats weighing 180±20g were randomly divided into four groups including: (1).control, (2).sham(saline), (3).hydrogen peroxide, (4).exercise & hydrogen peroxide. For induction of oxidative Stress,in the 3rd & 4th group 1mmol/kg H2O2 was injected to the rats intraperitoneally during 3 weeks every others days. In the exercise group, force swimming was conducted for 6-weeks, 60 minutes a day, 5-days a week. The activity of enzymes was measured by ELISA technic. Analysis of variance (ANOVA) was used to determine the significance.P<0.01 Results The results showed that induction of H2O2 caused a significant decrease in the activity of SOD & CAT, but resulted in a significant increase in activity of SOD enzyme in the heart tissue after six weeks of force swimming(P<0.01). The level of activity of the catalase was also increased, but this increase was not significant. Conclusion According to the findings of this study, forced swallowing significantly reduces the activity of the SOD enzyme by weakening the antioxidant defense system after the induction of H2O2. Therefore, this type of exercise can be used to strengthen the antioxidant system.

Keywords

• force swimming
• superoxide dismutase
• catalase
• hydrogen peroxide