Agronomy (Jan 2023)

Effects of Bioactive Composition in <i>Oryza sativa</i> L. cv. KDML105 Bran Extract on Gene Expression Related to Hair Cycle in Human Hair Follicle Dermal Papilla Cells

  • Chiranan Khantham,
  • Warintorn Ruksiriwanich,
  • Korawan Sringarm,
  • Chanakan Prom-u-thai,
  • Sansanee Jamjod,
  • Chaiwat Arjin,
  • Anurak Muangsanguan,
  • Pornchai Rachtanapun,
  • Kittisak Jantanasakulwong,
  • Yuthana Phimolsiripol,
  • Francisco J. Barba,
  • Sarana Rose Sommano,
  • Romchat Chutoprapat,
  • Korawinwich Boonpisuttinant

DOI
https://doi.org/10.3390/agronomy13020295
Journal volume & issue
Vol. 13, no. 2
p. 295

Abstract

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The aim of this study is to identify the effects of KDML105 bran extract on gene expression involving the hair cycle in HFDPCs and investigate its bioactive constituents, antioxidant, and anti-inflammatory activities. The content of tocopherols, γ-oryzanol, phytic acid, and phenolic compounds was quantified by liquid chromatography. Free fatty acids were determined using gas chromatography. Antioxidant capacities were estimated by DPPH, ABTS, and metal chelating assay. The nitric oxide (NO) production was determined by Griess reaction. Gene expression was measured by semi-quantitative RT-PCR. The major compounds in the extract were α- and γ-tocopherol, phytic acid, γ-oryzanol, chlorogenic acid, o-coumaric acid, palmitic acid, oleic acid, and linoleic acid, giving its antioxidant capacities. The nitrite level in lipopolysaccharide-induced macrophages (2.76 ± 0.13 μM) was significantly mitigated by the extract (0.81 ± 0.11 μM). Additionally, SRD5A2 and TGFB1 expressions in HFDPCs were downregulated, whereas CTNNB1 and VEGF genes were upregulated after treatment with the extract. KDML105 extract ameliorated oxidative stress and NO production. According to the gene expression study, KDML105 bran extract may be involved in the induction and maintenance of the anagen phase and angiogenesis in the hair growth pathway. Therefore, KDML105 bran extract might be a promising source of anti-hair loss substances.

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