Whole genome sequence of the emerging oomycete pathogen Pythium insidiosum strain CDC-B5653 isolated from an infected human in the USA
Marina S. Ascunce,
Jose C. Huguet-Tapia,
Edward L. Braun,
Almudena Ortiz-Urquiza,
Nemat O. Keyhani,
Erica M. Goss
Affiliations
Marina S. Ascunce
Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Plant Pathology, University of Florida, Gainesville, FL, United States
Jose C. Huguet-Tapia
Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States
Edward L. Braun
Department of Biology and Genetics Institute, University of Florida, Gainesville, FL, United States
Almudena Ortiz-Urquiza
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, United States
Nemat O. Keyhani
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, United States
Erica M. Goss
Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Plant Pathology, University of Florida, Gainesville, FL, United States; Corresponding author at: Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
Pythium insidiosum ATCC 200269 strain CDC-B5653, an isolate from necrotizing lesions on the mouth and eye of a 2-year-old boy in Memphis, Tennessee, USA, was sequenced using a combination of Illumina MiSeq (300 bp paired-end, 14 millions reads) and PacBio (10 Kb fragment library, 356,001 reads). The sequencing data were assembled using SPAdes version 3.1.0, yielding a total genome size of 45.6 Mb contained in 8992 contigs, N50 of 13 Kb, 57% G + C content, and 17,867 putative protein-coding genes. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JRHR00000000. Keywords: Oomycete, Pythium insidiosum, Pythiosis, Human emerging pathogen, Genome sequencing
