Tissue Distribution of Lipoprotein Lipase (LPL) and Regulation of LPL Gene Expression Induced by Insulin and Glucose in Goose Primary Hepatocytes

Abstract

The study was to research the gene expression of lipoprotein lipase (LPL) in abdomen adipose tissue, subcutaneous adipose tissue, pectoral muscle, skeletal muscles, liver, brain and spleen of geese, and the effect of insulin and glucose on gene expression of LPL was measured in primary hepatocytes of Sichuan white geese (Anser cygnoides) and Landes geese (Anser anser). We quantified gene expression by competitive reverse transcriptase PCR (RT-PCR). The results indicated: First, 0-150nM insulin could increase the mRNA level of LPL in a dose-dependent pattern, and 200nM insulin had an inhibiting effect. Second, there was a synergistic effect of insulin and glucose on LPL gene expression, and the synergistic effect of 30mM glucose and 50nM insulin was much higher than the single effect of insulin or glucose. In addition, the induction of LPL mRNA level by insulin and glucose was higher in Landes goose than the induction in Sichuan white goose. It was concluded that LPL was highly expressed in the goose liver, and the LPL gene level could be up-regulated by insulin and glucose.

Keywords

• gene expression
• glucose
• insulin
• lipoprotein lipase
• tissue distribution