Improvement of maturation and conversion of horse chestnut androgenic embryos

Abstract

Horse chestnut (Aesculus hippocastanum L., Hippocastanaceae) is a relict species of the tertiary flora and endemit of Balkan peninsula. It has enormous horticultular and medical important. Horse chestnut trees are native to the Balkan peninsula, but grow as ornamental trees in parks and avenues throughout the Northern Hemisphere. Because of the slow and difficult reproduction of great importance to be fast and cheap in vitro multiplication. Possible solution is regenerated by androgenesis. Microspore culture has been used in recent years as a tool for producing haploid plants in a varyety of higher plants, but the low frequencies of microspore-derived plants restrict the use of the technique in plant breeding.

Keywords

• Aesculus hippocastanum
• anther culture
• maturation
• ploidy level
• suspension culture