Chitosan loaded plant essential oils efficiently eradicate the multi-drug resistant bacterial infection and lung cancer cells

Abstract

In present research, of chitosan loaded plant essential oils (CsEOs) were performed against Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E.coli), K. pneumoniae and Staphylococci aureus (S. aureus), also the anti-cancer properties were evaluated against A549 lung cancer cells. Consequently, the CsEOs against P. aeruginosa, E.coli, K. pneumoniae and Staphylococci aureus were shown 24 mm, 22 mm, and 26 mm and 22 mm zones of inhibition respectively. In addition, 500 µg/mL of CsEOs exhibited 92, 89, 88 and 94% of inhibition against P. aeruginosa, E.coli, K. pneumoniae and S. aureus were observed respectively using micro broth dilution broth experiment. Further, cytotoxicity ability of CsEOs against A549 human lung cancer cells were monitored by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, and result of 500 µg/mL concentration was exhibited 58% inhibition against A549 lung cancer cells. Furthermore, the microscopic evidence of 500 µg/mL concentrations of CsEOs treated A549 lung cancer cells was showed irregular morphology in phase contrast microscope. Altogether, both the invitro and morphological experiments of anti-bacterial and anti-cancer study results were conveyed that the CsEOs have excellent anti-bacterial and anti-cancer properties. Finally, the entire study was proposed, the chosen CsEOs was excellent bionanomaterial used to deliver the drugs and bioactive compounds in target sites for inhibit the infectious pathogens and cancer cells.

Keywords

• Chitosan
• Essential oils
• Encapsulation
• Invitro inhibition study
• Anti-microbial activity
• Cytotoxicity assay