Viscoelasticity, Mechanical Properties, and In Vitro Bioactivity of Gelatin/Borosilicate Bioactive Glass Nanocomposite Hydrogels as Potential Scaffolds for Bone Regeneration

Abstract

Chemical cross-linking was used to create nanocomposite hydrogels made up of gelatin (G) and borosilicate bioactive glass (BBG) with different content (0, 3, and 5 wt.%). The G/BBG nanocomposite hydrogels were studied for their morphology, mechanical properties, and viscoelasticity. SEM images revealed a macroporous interconnected structure with particles scattered across the pore walls. Studies of water absorption and degradation confirmed that the nanocomposite scaffolds were hydrophilic and biodegradable. The addition of 5% BBG to the scaffold formulations increased the compressive modulus by 413% and the compressive intensity by 20%, respectively. At all frequency ranges tested, the storage modulus (G′) was greater than the loss modulus (G″), revealing a self-standing elastic nanocomposite hydrogel. The nanocomposite scaffolds facilitated apatite formation while immersed in simulated body fluid (SBF). According to the findings, G/BBG nanocomposite scaffolds could be a promising biomaterial for bone regeneration.

Keywords

• hydrogel
• gelatin
• borosilicate bioactive glass
• composite scaffold
• viscoelasticity
• bone regeneration