Nature Communications (May 2022)
Structure of the Arabidopsis guard cell anion channel SLAC1 suggests activation mechanism by phosphorylation
- Yawen Li,
- Yinan Ding,
- Lili Qu,
- Xinru Li,
- Qinxuan Lai,
- Pingxia Zhao,
- Yongxiang Gao,
- Chengbin Xiang,
- Chunlei Cang,
- Xin Liu,
- Linfeng Sun
Affiliations
- Yawen Li
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Yinan Ding
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Lili Qu
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Xinru Li
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Qinxuan Lai
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Pingxia Zhao
- School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, The Innovation Academy of Seed Design, Chinese Academy of Sciences
- Yongxiang Gao
- High-End Cryo-EM Platform, Core Facility Center for Life Sciences, University of Science and Technology of China
- Chengbin Xiang
- School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, The Innovation Academy of Seed Design, Chinese Academy of Sciences
- Chunlei Cang
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Xin Liu
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- Linfeng Sun
- Department of Neurology, the First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, CAS Centre for Excellence in Molecular Cell Science, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China
- DOI
- https://doi.org/10.1038/s41467-022-30253-3
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 9
Abstract
The anion channel SLAC1 controls stomatal closure upon phosphoactivation. Here via structural analysis and electrophysiology, the authors propose an inhibition-release model where phosphorylation causes dissociation of a cytosolic plug from the SLAC1 transmembrane domains to induce conformational change in the pore-forming helices.
