Comparison of two DNA extraction methods from larvae, pupae, and adults of Aedes aegypti
Josué M. de la Cruz-Ramos,
Luis M. Hernández-Triana,
Cristina García-De la Peña,
Vicente H. González-Álvarez,
James Weger-Lucarelli,
Quetzaly Karmy Siller-Rodríguez,
Francisco J. Sánchez Rámos,
Américo D. Rodríguez,
Aldo I. Ortega-Morales
Affiliations
Josué M. de la Cruz-Ramos
Universidad Autónoma Agraria Antonio Narro Unidad Laguna, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico; Unidad de Investigaciones Entomológicas y de Bioensayos del estado de Durango, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico
Luis M. Hernández-Triana
Animal and Plant Health Agency, Virology Department, Wildlife Zoonoses and Vector Borne Diseases Research Group, Woodham Lane, New Haw, Addlestone, Surrey, KT153NB, UK
Cristina García-De la Peña
Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Av. Universidad, 35010, Gómez Palacio, Durango, Mexico
Vicente H. González-Álvarez
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciajinicuilapa, Guerrero, Mexico
James Weger-Lucarelli
Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
Quetzaly Karmy Siller-Rodríguez
Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Av. Universidad, 35010, Gómez Palacio, Durango, Mexico
Francisco J. Sánchez Rámos
Universidad Autónoma Agraria Antonio Narro Unidad Laguna, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico
Américo D. Rodríguez
Centro Regional de Investigación en Salud Publica, Instituto Nacional de Salud Publica, Tapachula, Chiapas, Mexico
Aldo I. Ortega-Morales
Universidad Autónoma Agraria Antonio Narro Unidad Laguna, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico; Unidad de Investigaciones Entomológicas y de Bioensayos del estado de Durango, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico; Corresponding author.
Mosquitoes are the most important arthropods from the point of view of public health, due to the fact that they can transmit a large number of pathogens which can cause diseases to humans and animals. Aedes aegypti (L.) is one of the most important vector species in the world, since it can transmit numerous pathogens such as dengue, Zika, and chikungunya. Therefore, studies involving the molecular aspects of this and other mosquitoes species are currently increasing. In this report, we describe the comparison between two DNA extraction techniques, Chelex and cetyltrimethylammonium bromide (CTAB), for carrying out DNA extraction in larvae, pupae and adult female of Ae. aegypti. The Chelex technique was superior in the amount and purity of DNA as compared to the CTAB technique in the three life stages we tested.
