Production of Hyaluronic Acid by Streptococcus zooepidemicus on Protein Substrates Obtained from Scyliorhinus canicula Discards
José A. Vázquez,
Lorenzo Pastrana,
Carmen Piñeiro,
José A. Teixeira,
Ricardo I. Pérez-Martín,
Isabel R. Amado
Affiliations
José A. Vázquez
Grupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6. Vigo-36208 Galicia, Spain
Lorenzo Pastrana
Departamento de Química Analítica y Alimentaria, Facultad de Ciencias de Ourense (Universidad de Vigo), Campus As Lagoas s/n, Ourense-32004 Galicia, Spain
Carmen Piñeiro
Servicio de Instrumentación Científica (SICIM), Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6. Vigo-36208 Galicia, Spain
José A. Teixeira
Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal
Ricardo I. Pérez-Martín
Grupo de Bioquímica de Alimentos, Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6. Vigo-36208 Galicia, Spain
Isabel R. Amado
Departamento de Química Analítica y Alimentaria, Facultad de Ciencias de Ourense (Universidad de Vigo), Campus As Lagoas s/n, Ourense-32004 Galicia, Spain
This work investigates the production of hyaluronic acid (H) by Streptococcus equi subsp. zooepidemicus in complex media formulated with peptones obtained from Scyliorhinus canicula viscera by-products. Initially, in batch cultures, the greatest productions were achieved using commercial media (3.03 g/L) followed by peptones from alcalase hydrolyzed viscera (2.32 g/L) and peptones from non-hydrolyzed viscera (2.26 g/L). An increase of between 12% and 15% was found in subsequent fed-batch cultures performed on waste peptones. Such organic nitrogen sources were shown to be an excellent low-cost substrate for microbial H, saving more than 50% of the nutrient costs.
