INVESTIGATION OF CHRONIC TOXICITY OF A PHARMACOLOGICAL COMPOSITION BASED ON BACTERIOCIN-NISIN AND SILICON GLYCEROLATES

Abstract

The use of new drugs on productive animals is possible only after identifying the long-term effects of the use of substances included in the composition on the functional state of organs, tissues and systems. As a result, the goal of our work was to study the chronic toxicity of the developed composition and the possibility of using it on highly productive cows in the treatment of mastitis. The studied composition was developed at the I.Ya. Postovsky Insititute of Organic Synthesis of the Ural Branch of the Russian Academy of Sciencesand corresponds to the composition: silicon glycerolates – 3,0%, nisin – 0,3%, glycerin – 10%, distilled water – up to 100 %. Chronic toxicity studies were performed on female non-linear white laboratory rats (n=40) using hematological, biochemical, pathomorphological and histological research methods. When conducting hematological studies, it was found that the indicators of the composition of the blood elements of laboratory rats when using the studied composition in different dosages were within the limits. A study of the biochemical parameters of the blood of laboratory animals receiving the composition at a dose of 500 mg/kg and 1000 mg / kg found that the metabolic parameters corresponded to physiological values. Feeding of the studied composition at a dose of 750 mg / kg led to changes in the form of an increase in the enzymatic activity of alkaline phosphatase, a decrease in cholesterol, however, the data obtained cannot confirm the negative effect of the studied dose on the liver, due to the absence of pathological changes in this organ at the cellular level. Histological studies have established that the developed composition can cause changes in organs depending on the dosage used. Based on the research results, we determined that the composition can be recommended for clinical trials on productive animal species at a dose of no more than 500 mg/kg. The use of a dose of 750 mg / kg is recommended to be used with caution in animals with severe liver dysfunction. During further tests, it is necessary to refrain from the critical dose of 1000 mg / kg, which leads to deeper changes in the organs.

Keywords

• chronic toxicity
• laboratory animals
• medicinal composition
• bacteriocin-nisin
• silicon glycerolates