PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO
Revista Colombiana de Química. 2011;41(1):31-45
Journal Title: Revista Colombiana de Química
ISSN: 0120-2804 (Print); 2357-3791 (Online)
Publisher: Universidad Nacional de Colombia
Society/Institution: Universidad Nacional de Colombia, Departamento de Quimica
LCC Subject Category: Science: Chemistry
Country of publisher: Colombia
Language of fulltext: English, Spanish; Castilian, Portuguese
Full-text formats available: PDF, HTML, XML
AUTHORS
Edgar Antonio Reyes Montaño
(Universidad Nacional de Colombia)
Leonardo Lareo
(Pontificia Universidad Javeriana)
Gerardo Pérez
(Universidad Nacional de Colombia)
EDITORIAL INFORMATION
Time From Submission to Publication: 12 weeks
Abstract | Full Text
<div>Producing polyclonal antibodies (IgY) in</div><div>chickens has advantages over those obtained</div><div>in other animal models, since they</div><div>have been used as a tool for studying</div><div>different proteins (NMDA glutamate receptor</div><div>in our case, specifically the NR1</div><div>subunit). We produced specific antibodies</div><div>against expression products by the</div><div>alternative splicing of the gene encoding</div><div>NMDA receptor NR1 subunit in adult rat</div><div>brain. Three peptides corresponding to</div><div>the splicing sites (N1, C1 and C2’ cassettes)</div><div>were designed, synthesised and used</div><div>individually as antigens in hens. Specific</div><div>immunoglobulins were purified from</div><div>yolks. The antibodies were then used for</div><div>purifying the NMDA receptor NR1 subunit</div><div>using affinity chromatography coupling</div><div>the three antibodies to the support.R</div>