PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO

Revista Colombiana de Química. 2011;41(1):31-45

 

Journal Homepage

Journal Title: Revista Colombiana de Química

ISSN: 0120-2804 (Print); 2357-3791 (Online)

Publisher: Universidad Nacional de Colombia

Society/Institution: Universidad Nacional de Colombia, Departamento de Quimica

LCC Subject Category: Science: Chemistry

Country of publisher: Colombia

Language of fulltext: Portuguese, Spanish; Castilian, English

Full-text formats available: PDF, HTML, XML

 

AUTHORS

Edgar Antonio Reyes Montaño (Universidad Nacional de Colombia)
Leonardo Lareo (Pontificia Universidad Javeriana)
Gerardo Pérez (Universidad Nacional de Colombia)

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 12 weeks

 

Abstract | Full Text

<div>Producing polyclonal antibodies (IgY) in</div><div>chickens has advantages over those obtained</div><div>in other animal models, since they</div><div>have been used as a tool for studying</div><div>different proteins (NMDA glutamate receptor</div><div>in our case, specifically the NR1</div><div>subunit). We produced specific antibodies</div><div>against expression products by the</div><div>alternative splicing of the gene encoding</div><div>NMDA receptor NR1 subunit in adult rat</div><div>brain. Three peptides corresponding to</div><div>the splicing sites (N1, C1 and C2’ cassettes)</div><div>were designed, synthesised and used</div><div>individually as antigens in hens. Specific</div><div>immunoglobulins were purified from</div><div>yolks. The antibodies were then used for</div><div>purifying the NMDA receptor NR1 subunit</div><div>using affinity chromatography coupling</div><div>the three antibodies to the support.R</div>