Versatile protein tagging in cells with split fluorescent protein

Daichi Kamiyama; Sayaka Sekine; Benjamin Barsi-Rhyne; Jeffrey Hu; Baohui Chen; Luke A. Gilbert; Hiroaki Ishikawa; Manuel D. Leonetti; Wallace F. Marshall; Jonathan S. Weissman; Bo Huang

doi:10.1038/ncomms11046

Nature Communications (Mar 2016)

Versatile protein tagging in cells with split fluorescent protein

Daichi Kamiyama,
Sayaka Sekine,
Benjamin Barsi-Rhyne,
Jeffrey Hu,
Baohui Chen,
Luke A. Gilbert,
Hiroaki Ishikawa,
Manuel D. Leonetti,
Wallace F. Marshall,
Jonathan S. Weissman,
Bo Huang

Affiliations

Daichi Kamiyama: Department of Pharmaceutical Chemistry, University of California
Sayaka Sekine: Department of Pharmaceutical Chemistry, University of California
Benjamin Barsi-Rhyne: Tetrad Graduate Program, University of California
Jeffrey Hu: Department of Pharmaceutical Chemistry, University of California
Baohui Chen: Department of Pharmaceutical Chemistry, University of California
Luke A. Gilbert: Department of Cellular and Molecular Pharmacology, University of California
Hiroaki Ishikawa: Department of Biochemistry and Biophysics, University of California
Manuel D. Leonetti: Department of Cellular and Molecular Pharmacology, University of California
Wallace F. Marshall: Department of Biochemistry and Biophysics, University of California
Jonathan S. Weissman: Department of Cellular and Molecular Pharmacology, University of California
Bo Huang: Department of Pharmaceutical Chemistry, University of California

DOI: https://doi.org/10.1038/ncomms11046
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 9

Abstract

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Tagging proteins with fluorescent proteins is a powerful method for both imaging and non-imaging applications. Here the authors use the eleventh β-strand of sfGFP and sfCherry as epitope tags for multicolour imaging and amplified signals by tandem arrangement; shortness of the tag enabled introduction into genomic loci using CRISPR/Cas9.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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