Enzymatic synthesis of cytidine diphosphate diglyceride

Abstract

Evidence is presented for the enzymatic formation of cytidine diphosphate diglyceride in microsomal preparations from guinea pig liver according to the reaction:CTP + phosphatidic acid ⇌ CDP-diglyceride + P—O—P.Conditions have been found in which the incorporation of labeled CTP into CDP-diglyceride is almost entirely dependent upon added phosphatidic acid. The incorporation of CMP into lipid is very slight. A substantial net synthesis of CDP-diglyceride takes place under these conditions. Some properties of the enzyme system are described.

Keywords

• cytidine nucleotide
• CDP-diglyceride
• enzymatic
• biosynthesis
• phosphatidic acid
• microsomes