Preparation of Lir@BSA-PMF nanoparticles and verification of their cell functions

Abstract

Objective To synthesize bovine serum albumin(BSA)-loaded liraqlutide (Lir)-nanoparticles coated with platelet membrane fragments (PMF) using a“bottom-up” nano-engineering chemistry technique, and to evaluate their cyto-compatibility and potential function of anti-oxidative stress. Methods PMF was extracted as reported previously. Lir@BSA nanoparticles were prepared by self-assembly method. PMF was coated on the surface of Lir@BSA nanoparticles by co-extrusion to prepare Lir@BSA-PMF. The physical and chemical properties of Lir@BSA-PMF particles were characterized as particle size, Zeta potential, transmission electron microscopy and particle size stability. The encapsulation efficiency, loading efficiency and cumulative release efficiency of liraglutide were calculated by enzyme-linked immunosorbent assay. Further, SDS-PAGE was used to analyze whether there was a similar membrane protein distribution of platelet membrane on Lir@BSA-PMF bionicnanocarrier.CCK-8 assay was used to verify the biocompatibility of the materials. Reactive oxygen species (ROS) experiment was used to explore the effect of Lir@BSA-PMF on cell oxidative damage. The uptake of cells on Lir@BSA-PMF bionic nano capsules was verified by cell phagocytosis experiment. Results Lir@BSA-PMF nanoparticles had a stable particle size of 25 nm with a spherical morphology, and a Zeta potential value of -25.5 mV. The encapsulation efficiency, loading efficiency and cumulative release efficiency of liraglutide were 85.56%, 7.96% and 77.06%, respectively. SDS-PAGE analysis showed that the Lir@BSA-PMF bio-mimetic nano capsules retained the similar membrane protein distribution as platelet membrane. CCK-8 assay verified that the nanomaterials were non-cytotoxic. ROS results showed that Lir@BSA-PMF nanomaterials had obvious antioxidant properties. The results of cell phagocytosis showed that the cells had a good phagocytosis effect on Lir@BSA-PMF nanoparticles. Conclusions The nanoparticles Lir@BSA-PMF are successfully synthesized and have no effects on cells viability in vitro. The particles are taken up by cells and show a significant function of antioxidant damage.

Keywords

• platelet membrane fragments|liraglutide|nanoparticles|cell functions