Bacillus cereus NJ01 induces SA- and ABA-mediated immunity against bacterial pathogens through the EDS1-WRKY18 module

Dacheng Wang; Lirong Wei; Jinbiao Ma; Yingqiao Wan; Keyi Huang; Yiqiong Sun; Huili Wen; Zhipeng Chen; Zijie Li; Dongli Yu; Haitao Cui; Jingni Wu; Yufeng Wu; Sun Tae Kim; Jing Zhao; Jane E. Parker; Kenichi Tsuda; Chunhao Jiang; Yiming Wang

Cell Reports (Apr 2024)

Bacillus cereus NJ01 induces SA- and ABA-mediated immunity against bacterial pathogens through the EDS1-WRKY18 module

Dacheng Wang,
Lirong Wei,
Jinbiao Ma,
Yingqiao Wan,
Keyi Huang,
Yiqiong Sun,
Huili Wen,
Zhipeng Chen,
Zijie Li,
Dongli Yu,
Haitao Cui,
Jingni Wu,
Yufeng Wu,
Sun Tae Kim,
Jing Zhao,
Jane E. Parker,
Kenichi Tsuda,
Chunhao Jiang,
Yiming Wang

Affiliations

Dacheng Wang: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Lirong Wei: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Jinbiao Ma: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Yingqiao Wan: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Keyi Huang: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Yiqiong Sun: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Huili Wen: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Key Laboratory for Information Agriculture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China
Zhipeng Chen: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Key Laboratory for Information Agriculture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China
Zijie Li: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Dongli Yu: Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Haitao Cui: Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China
Jingni Wu: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Yufeng Wu: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Key Laboratory for Information Agriculture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China
Sun Tae Kim: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea
Jing Zhao: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Jane E. Parker: Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany
Kenichi Tsuda: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Corresponding author
Chunhao Jiang: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author
Yiming Wang: Department of Plant Pathology, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author

Journal volume & issue: Vol. 43, no. 4
p. 113985

Abstract

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Summary: Emerging evidence suggests a beneficial role of rhizobacteria in ameliorating plant disease resistance in an environment-friendly way. In this study, we characterize a rhizobacterium, Bacillus cereus NJ01, that enhances bacterial pathogen resistance in rice and Arabidopsis. Transcriptome analyses show that root inoculation of NJ01 induces the expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes in Arabidopsis leaves. Genetic evidence showed that EDS1, PAD4, and WRKY18 are required for B. cereus NJ01-induced bacterial resistance. An EDS1-PAD4 complex interacts with WRKY18 and enhances its DNA binding activity. WRKY18 directly binds to the W box in the promoter region of the SA biosynthesis gene ICS1 and ABA biosynthesis genes NCED3 and NCED5 and contributes to the NJ01-induced bacterial resistance. Taken together, our findings indicate a role of the EDS1/PAD4-WRKY18 complex in rhizobacteria-induced disease resistance.

CP: Plants

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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