A procedure for the preparation and isolation of nucleoside-5’-diphosphates

Abstract

Tris[bis(triphenylphosphoranylidene)ammonium] pyrophosphate (PPN pyrophosphate) was used in the SN2 displacements of the tosylate ion from 5’-tosylnucleosides to afford nucleoside-5’-diphosphates. Selective precipitation permitted the direct isolation of nucleoside-5’-diphosphates from crude reaction mixtures.

Keywords

• NDP synthesis
• nucleic acids
• nucleoside-5’-diphosphate
• phosphorylation