Scientific Reports (May 2022)

Deep learning for blind structured illumination microscopy

  • Emmanouil Xypakis,
  • Giorgio Gosti,
  • Taira Giordani,
  • Raffaele Santagati,
  • Giancarlo Ruocco,
  • Marco Leonetti

Journal volume & issue
Vol. 12, no. 1
pp. 1 – 7


Read online

Abstract Blind-structured illumination microscopy (blind-SIM) enhances the optical resolution without the requirement of nonlinear effects or pre-defined illumination patterns. It is thus advantageous in experimental conditions where toxicity or biological fluctuations are an issue. In this work, we introduce a custom convolutional neural network architecture for blind-SIM: BS-CNN. We show that BS-CNN outperforms other blind-SIM deconvolution algorithms providing a resolution improvement of 2.17 together with a very high Fidelity (artifacts reduction). Furthermore, BS-CNN proves to be robust in cross-database variability: it is trained on synthetically augmented open-source data and evaluated on experiments. This approach paves the way to the employment of CNN-based deconvolution in all scenarios in which a statistical model for the illumination is available while the specific realizations are unknown or noisy.