Preparation process by desolvation method for enhanced loading of acyclovir nanoparticles

Abstract

The aim of this investigation was to qualitatively study on preparation process of enhanced loading acyclovir (ACV) in ACV-loaded bovine serum albumin (BSA) prepared by desolvation method with submerged jet of desolvating agent. The prepared ACV-loaded BSA nanoparticles in sterile water for injection (SWI) and isotonic trehalose solution were shown to be monodisperse with sizes of around 120 to 200 nm and zeta potentials of around -7 to -50 mV. However, those in phosphate buffer saline (PBS) were found to exhibit much larger sizes with polydispersity, which might be attributed to the effect of ionic strength. The loading efficiency was found to be around 60%. An increase in the amount of ACV added to the system could significantly improve the loading capacity by almost the same ratio, which may be due to molecular mixing behavior of submerged jet of desolvating agent.