mSphere
(Aug 2021)
Mutagenesis Mapping of RNA Structures within the Foot-and-Mouth Disease Virus Genome Reveals Functional Elements Localized in the Polymerase (3D
<sup>pol</sup>
)-Encoding Region
Lidia Lasecka-Dykes,
Fiona Tulloch,
Peter Simmonds,
Garry A. Luke,
Paolo Ribeca,
Sarah Gold,
Nick J. Knowles,
Caroline F. Wright,
Jemma Wadsworth,
Mehreen Azhar,
Donald P. King,
Tobias J. Tuthill,
Terry Jackson,
Martin D. Ryan
Affiliations
Lidia Lasecka-Dykes
ORCiD The Pirbright Institute, Pirbright, Surrey, United Kingdom
Fiona Tulloch
Biomedical Sciences Research Complex (BSRC), School of Biology, University of St. Andrews, St. Andrews, United Kingdom
Peter Simmonds
ORCiD Nuffield Department of Experimental Medicine, University of Oxford, Oxford, United Kingdom
Garry A. Luke
Biomedical Sciences Research Complex (BSRC), School of Biology, University of St. Andrews, St. Andrews, United Kingdom
Paolo Ribeca
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Sarah Gold
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Nick J. Knowles
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Caroline F. Wright
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Jemma Wadsworth
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Mehreen Azhar
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Donald P. King
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Tobias J. Tuthill
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Terry Jackson
The Pirbright Institute, Pirbright, Surrey, United Kingdom
Martin D. Ryan
Biomedical Sciences Research Complex (BSRC), School of Biology, University of St. Andrews, St. Andrews, United Kingdom
DOI
https://doi.org/10.1128/mSphere.00015-21
Journal volume & issue
Vol. 6,
no. 4
Abstract
Read online
Some RNA structures formed by the genomes of RNA viruses are critical for viral replication. Our study shows that of 46 conserved RNA structures located within the regions of the foot-and-mouth disease virus (FMDV) genome that encode the nonstructural proteins, only 3 are essential for replication of an FMDV subgenomic replicon.
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