Evaluation of Virgibacillus dokdonensis MCCC 1A00493 as a biological control agent and microbial organic fertilizer against root-knot nematodes

Wen Chen; Zhicheng Zhu; Chen Liu; Fan Yang; Wei Dai; Hao Yu; Dian Huang; Wanli Cheng; Zongze Shao; Kashif ur Rehman; Jibin Zhang

Biological Control (May 2024)

Evaluation of Virgibacillus dokdonensis MCCC 1A00493 as a biological control agent and microbial organic fertilizer against root-knot nematodes

Wen Chen,
Zhicheng Zhu,
Chen Liu,
Fan Yang,
Wei Dai,
Hao Yu,
Dian Huang,
Wanli Cheng,
Zongze Shao,
Kashif ur Rehman,
Jibin Zhang

Affiliations

Wen Chen: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Zhicheng Zhu: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Chen Liu: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Fan Yang: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Wei Dai: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Hao Yu: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Dian Huang: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Wanli Cheng: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan 430062, Hubei, China
Zongze Shao: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
Kashif ur Rehman: German Institute of Food Technologies (DIL e.V.), Prof.-v.-Klitzing-Str. 7, 49610 Quakenbrück, Germany; Department of Microbiology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
Jibin Zhang: National Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbe Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Corresponding author.

Journal volume & issue: Vol. 192
p. 105508

Abstract

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Root-knot nematodes (RKNs) can result in severe losses to crop production and economies. Virgibacillus dokdonensis MCCC 1A00493, a deep-sea bacterium, has demonstrated potent antagonistic effects against Meloidogyne incognita in vitro. However, the control efficacy under greenhouse and field conditions remains unclear. In this study, we investigated the nematicidal effect of MCCC 1A00493 fermentation liquid and its synergism with black soldier fly (BSF) larval frass organic fertilizer both in greenhouse and field experiments. MCCC 1A00493 fermentation liquid demonstrated a significant control efficacy on RKNs both in greenhouse and field experiments, with the highest control efficacy reaching 68.97 % and 74.87 %, respectively. Meanwhile, the synergistic effects of MCCC 1A00493 and BSF larvae frass organic fertilizer was investigated by adding 10 % suspension (1 × 109 CFU/mL) of MCCC 1A00493 into 71.51 kg of chicken manure organic fertilizer produced by co-conversion of BSF larvae and Bacillus subtilis BSF-CL. It was noted that MCCC 1A00493 microbial organic fertilizer reduced disease incidence of RKNs significantly both in greenhouse and field experiments, with the root gall index decreasing by 51.75 % and 63.22 %, respectively. MCCC 1A00493 fermentation liquid and microbial organic fertilizer demonstrated a control efficacy on RKNs exceeded commercial nematicide avermectin and commercial bio-organic fertilizer, respectively. In addition, MCCC 1A00493 fermentation liquid and microbial organic fertilizer had positive effects on the growth of tomato plants. These findings confirmed that V.dokdonensis MCCC 1A00493 as a biological control agent and microbial organic fertilizer have significant effect on RKNs and can be used as potential biocontrol preparations.

Published in Biological Control

ISSN: 1049-9644 (Print); 1090-2112 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture; Science: Biology (General)
Website: https://www.sciencedirect.com/journal/biological-control

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