Nature Communications (Jul 2017)

Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

  • Ramunas Stepanauskas,
  • Elizabeth A. Fergusson,
  • Joseph Brown,
  • Nicole J. Poulton,
  • Ben Tupper,
  • Jessica M. Labonté,
  • Eric D. Becraft,
  • Julia M. Brown,
  • Maria G. Pachiadaki,
  • Tadas Povilaitis,
  • Brian P. Thompson,
  • Corianna J. Mascena,
  • Wendy K. Bellows,
  • Arvydas Lubys

DOI
https://doi.org/10.1038/s41467-017-00128-z
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 10

Abstract

Read online

Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmental samples.