Journal of Extracellular Vesicles (May 2021)
Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
- Uta Erdbrügger,
- Charles J. Blijdorp,
- Irene V. Bijnsdorp,
- Francesc E. Borràs,
- Dylan Burger,
- Benedetta Bussolati,
- James Brian Byrd,
- Aled Clayton,
- James W. Dear,
- Juan M. Falcón‐Pérez,
- Cristina Grange,
- Andrew F. Hill,
- Harry Holthöfer,
- Ewout J. Hoorn,
- Guido Jenster,
- Connie R. Jimenez,
- Kerstin Junker,
- John Klein,
- Mark A. Knepper,
- Erik H. Koritzinsky,
- James M. Luther,
- Metka Lenassi,
- Janne Leivo,
- Inge Mertens,
- Luca Musante,
- Eline Oeyen,
- Maija Puhka,
- Martin E. vanRoyen,
- Catherine Sánchez,
- Carolina Soekmadji,
- Visith Thongboonkerd,
- Volkert vanSteijn,
- Gerald Verhaegh,
- Jason P. Webber,
- Kenneth Witwer,
- Peter S.T. Yuen,
- Lei Zheng,
- Alicia Llorente,
- Elena S. Martens‐Uzunova
Affiliations
- Uta Erdbrügger
- Division of Nephrology University of Virginia Health System Charlottesville Virginia USA
- Charles J. Blijdorp
- Department of Internal medicine Division of Nephrology and Transplantation Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands
- Irene V. Bijnsdorp
- Department of Urology Amsterdam UMC location VUmc Cancer Center Amsterdam Amsterdam The Netherlands
- Francesc E. Borràs
- REMAR‐IVECAT Group, “Germans Trias i Pujol” Health Science Research Institute and Nephrology Department “Germans Trias i Pujol” University Hospital Badalona Spain
- Dylan Burger
- Kidney Research Centre Ottawa Hospital Research Institute and Department of Cellular and Molecular Medicine University of Ottawa Canada
- Benedetta Bussolati
- Department of Molecular Biotechnology and Health Science University of Torino Torino Italy
- James Brian Byrd
- Department of Internal Medicine University of Michigan Medical School Ann Arbor Michigan USA
- Aled Clayton
- Tissue Microenvironment Group Division of Cancer & Genetics School of Medicine Cardiff University United Kingdom
- James W. Dear
- Centre for Cardiovascular Science Queen's Medical Research Institute University of Edinburgh Edinburgh United Kingdom
- Juan M. Falcón‐Pérez
- Exosomes Laboratory and Metabolomics Platform Bizkaia Technology Park CIC bioGUNE‐BRTA Derio Spain
- Cristina Grange
- Department of Medical Sciences University of Torino Torino Italy
- Andrew F. Hill
- Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science La Trobe University Bundoora Victoria Australia
- Harry Holthöfer
- Internal Medicine University Clinic Hamburg‐Eppendorf Hamburg Germany
- Ewout J. Hoorn
- Department of Internal medicine Division of Nephrology and Transplantation Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands
- Guido Jenster
- Erasmus MC Cancer Institute Department of Urology Laboratory of Experimental Urology University Medical Center Rotterdam Erasmus MC Rotterdam The Netherlands
- Connie R. Jimenez
- Department of Urology Amsterdam UMC location VUmc Cancer Center Amsterdam Amsterdam The Netherlands
- Kerstin Junker
- Department of Urology Saarland University Medical Centre Homburg Germany
- John Klein
- Department of Medicine University of Louisville School of Medicine Louisville Kentucky USA
- Mark A. Knepper
- Epithelial Systems Biology Laboratory Systems Biology Center National Heart, Lung, and Blood Institute National Institutes of Health Bethesda Maryland USA
- Erik H. Koritzinsky
- Renal Diagnostics and Therapeutics Unit National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda Maryland USA
- James M. Luther
- Division of Clinical Pharmacology Department of Medicine Vanderbilt University Medical Center Nashville Tennessee USA
- Metka Lenassi
- Institute of Biochemistry Faculty of Medicine University of Ljubljana Ljubljana Slovenia
- Janne Leivo
- Department of Biochemistry University of Turku Turku Finland
- Inge Mertens
- Centre for Proteomics, University of Antwerp and Health Unit, VITO Antwerp Belgium
- Luca Musante
- Division of Nephrology University of Virginia Health System Charlottesville Virginia USA
- Eline Oeyen
- Centre for Proteomics, University of Antwerp and Health Unit, VITO Antwerp Belgium
- Maija Puhka
- Institute for Molecular Medicine Finland FIMM University of Helsinki Helsinki Finland
- Martin E. vanRoyen
- Department of Pathology Erasmus MC Cancer Institute University Medical Center Rotterdam Erasmus MC Rotterdam The Netherlands
- Catherine Sánchez
- Department of Urology Clínica las Condes Research Core Santiago Chile
- Carolina Soekmadji
- Department of Cell and Molecular Biology QIMR Berghofer Medical Research Institute Brisbane Australia
- Visith Thongboonkerd
- Medical Proteomics Unit Office for Research and Development Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand
- Volkert vanSteijn
- Department of Chemical Engineering TU Delft Delft The Netherlands
- Gerald Verhaegh
- Department of Urology Radboud University Medical Center Nijmegen The Netherlands
- Jason P. Webber
- Institute of Life Science 1 Swansea University Medical School Singleton Park Campus Swansea UK
- Kenneth Witwer
- Departments of Molecular and Comparative Pathobiology and Neurology John Hopkins University School of Medicine Baltimore Maryland USA
- Peter S.T. Yuen
- Renal Diagnostics and Therapeutics Unit National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda Maryland USA
- Lei Zheng
- Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou Guangdong China
- Alicia Llorente
- Department of Molecular Cell Biology Institute for Cancer Research Oslo University Hospital Oslo Norway
- Elena S. Martens‐Uzunova
- Erasmus MC Cancer Institute Department of Urology Laboratory of Experimental Urology University Medical Center Rotterdam Erasmus MC Rotterdam The Netherlands
- DOI
- https://doi.org/10.1002/jev2.12093
- Journal volume & issue
-
Vol. 10,
no. 7
pp. n/a – n/a
Abstract
Abstract Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV‐based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
Keywords
