Antioxidant and antimicrobial activities with GC/MS analysis of the Morus alba L. Leaves

Abstract

Morus alba L. Moraceae leaves are used effectively to treat fever, protect liver from damage, strengthen the joints, facilitate discharge of urine in Turkey folk medicine. In this study we aimed to determine in vitro antioxidant and antimicrobial activity with GC/MS analysis of the Morus alba L. leaves. Dried plant leaves samples are milled and extracted with distilled water and ethanol in Soxhlet machine. After extraction, extract samples were concentrated in rotary evaporator machine. Total antioxidant status values were determined as mmol Trolox Equivalent/L spectrophotometrically by using Erel’s method. Sterile extracts were used to avoid contamination in antimicrobial activity test. As test microorganisms Escherichia coli ATCC 25922 , Staphylococcus aureus ATCC 25923 , Enterococcus faecalis ATCC 29212 , Pseudomonas aeroginosa ATCC 27853 , Candida albicans ATCC 90028 were used. Antimicrobial activity was determined with disc diffusion method. We used ethanol extracts of samples in GC/MS analysis. Ethanol and distilled water extracts showed antioxidant activity in different rate. Besides, ethanol extracts have an antimicrobial activity but water extracts have not antimicrobial activity on our test microorganisms. We determined four compounds 9,12,15-octadecatrienoic acid, ethyl ester, linolenic acid ethyl ester, gibberellic acid with GC/MS analysis in ethanol extracts

Keywords

• antioxidant activity
• antimicrobial activity
• gc/ms analysis
• morus alba l.
• extraction