Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels
Anita Bányai,
Enikő Farkas,
Hajnalka Jankovics,
Inna Székács,
Eszter Leelőssyné Tóth,
Ferenc Vonderviszt,
Róbert Horváth,
Máté Varga,
Péter Fürjes
Affiliations
Anita Bányai
Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary
Enikő Farkas
Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary
Hajnalka Jankovics
Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem Str. 10, H-8200 Veszprém, Hungary
Inna Székács
Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary
Eszter Leelőssyné Tóth
Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary
Ferenc Vonderviszt
Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem Str. 10, H-8200 Veszprém, Hungary
Róbert Horváth
Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary
Máté Varga
77 Elektronika Ltd., Fehérvári Str. 98, H-1111 Budapest, Hungary
Péter Fürjes
Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary
The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (Ø = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 µm) at different flow rates (0.5, 1, 2 µL/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped Escherichia coli (E. coli), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, Saccharomyces cerevisiae, and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel.
