A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells

Robert Lu; Joshua A.M. Allen; Pablo Galaviz; Hilda A. Pickett

STAR Protocols (Mar 2022)

A DNA-fiber protocol for single molecule analysis of telomere (SMAT) length and extension events in cancer cells

Robert Lu,
Joshua A.M. Allen,
Pablo Galaviz,
Hilda A. Pickett

Affiliations

Robert Lu: Children’s Medical Research Institute, University of Sydney, Westmead, NSW 2145, Australia; Corresponding author
Joshua A.M. Allen: Children’s Medical Research Institute, University of Sydney, Westmead, NSW 2145, Australia
Pablo Galaviz: Children’s Medical Research Institute, University of Sydney, Westmead, NSW 2145, Australia
Hilda A. Pickett: Children’s Medical Research Institute, University of Sydney, Westmead, NSW 2145, Australia; Corresponding author

Journal volume & issue: Vol. 3, no. 1
p. 101212

Abstract

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Summary: Alternative lengthening of telomeres (ALT) is a homologous recombination-based telomere maintenance mechanism. It is active in approximately 10–15% of cancers. We present a DNA-fiber protocol, combining YOYO-1 staining of genomic DNA, telomere fluorescence in situ hybridization (FISH), and EdU labeling of nascent DNA, to measure telomere extension events in ALT cancer cells. The protocol can be used to delineate ALT-mediated telomere extension.For complete details on the use and execution of this protocol, please refer to Barroso-Gonzalez et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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