eLife (Feb 2019)
Arabidopsis RCD1 coordinates chloroplast and mitochondrial functions through interaction with ANAC transcription factors
- Alexey Shapiguzov,
- Julia P Vainonen,
- Kerri Hunter,
- Helena Tossavainen,
- Arjun Tiwari,
- Sari Järvi,
- Maarit Hellman,
- Fayezeh Aarabi,
- Saleh Alseekh,
- Brecht Wybouw,
- Katrien Van Der Kelen,
- Lauri Nikkanen,
- Julia Krasensky-Wrzaczek,
- Nina Sipari,
- Markku Keinänen,
- Esa Tyystjärvi,
- Eevi Rintamäki,
- Bert De Rybel,
- Jarkko Salojärvi,
- Frank Van Breusegem,
- Alisdair R Fernie,
- Mikael Brosché,
- Perttu Permi,
- Eva-Mari Aro,
- Michael Wrzaczek,
- Jaakko Kangasjärvi
Affiliations
- Alexey Shapiguzov
- ORCiD
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland; Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
- Julia P Vainonen
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
- Kerri Hunter
- ORCiD
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
- Helena Tossavainen
- Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, Finland; Department of Chemistry, Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland
- Arjun Tiwari
- Department of Biochemistry / Molecular Plant Biology, University of Turku, Turku, Finland
- Sari Järvi
- Department of Biochemistry / Molecular Plant Biology, University of Turku, Turku, Finland
- Maarit Hellman
- Department of Chemistry, Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland
- Fayezeh Aarabi
- Max-Planck Institute for Molecular Plant Physiology, Potsdam, Germany
- Saleh Alseekh
- ORCiD
- Max-Planck Institute for Molecular Plant Physiology, Potsdam, Germany; Center of Plant System Biology and Biotechnology, Plovdiv, Bulgaria
- Brecht Wybouw
- ORCiD
- Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium
- Katrien Van Der Kelen
- Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium
- Lauri Nikkanen
- Department of Biochemistry / Molecular Plant Biology, University of Turku, Turku, Finland
- Julia Krasensky-Wrzaczek
- ORCiD
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
- Nina Sipari
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Metabolomics Unit, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
- Markku Keinänen
- Department of Environmental and Biological Sciences, University of Eastern Finland, Joensuu, Finland
- Esa Tyystjärvi
- Department of Biochemistry / Molecular Plant Biology, University of Turku, Turku, Finland
- Eevi Rintamäki
- Department of Biochemistry / Molecular Plant Biology, University of Turku, Turku, Finland
- Bert De Rybel
- ORCiD
- Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium
- Jarkko Salojärvi
- ORCiD
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
- Frank Van Breusegem
- Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium; VIB Center for Plant Systems Biology, Ghent, Belgium
- Alisdair R Fernie
- Max-Planck Institute for Molecular Plant Physiology, Potsdam, Germany; Center of Plant System Biology and Biotechnology, Plovdiv, Bulgaria
- Mikael Brosché
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland; Institute of Technology, University of Tartu, Tartu, Estonia
- Perttu Permi
- Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, Finland; Department of Chemistry, Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland; Department of Biological and Environmental Science, Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland
- Eva-Mari Aro
- Department of Biochemistry / Molecular Plant Biology, University of Turku, Turku, Finland
- Michael Wrzaczek
- ORCiD
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
- Jaakko Kangasjärvi
- ORCiD
- Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, Helsinki, Finland
- DOI
- https://doi.org/10.7554/eLife.43284
- Journal volume & issue
-
Vol. 8
Abstract
Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo and suppresses the activity of the transcription factors ANAC013 and ANAC017, which mediate a ROS-related retrograde signal originating from mitochondrial complex III. Inactivation of RCD1 leads to increased expression of mitochondrial dysfunction stimulon (MDS) genes regulated by ANAC013 and ANAC017. Accumulating MDS gene products, including alternative oxidases (AOXs), affect redox status of the chloroplasts, leading to changes in chloroplast ROS processing and increased protection of photosynthetic apparatus. ROS alter the abundance, thiol redox state and oligomerization of the RCD1 protein in vivo, providing feedback control on its function. RCD1-dependent regulation is linked to chloroplast signaling by 3'-phosphoadenosine 5'-phosphate (PAP). Thus, RCD1 integrates organellar signaling from chloroplasts and mitochondria to establish transcriptional control over the metabolic processes in both organelles.
Keywords
