Studies on recent IBD virus field variant isolates:Genomic identification and differentiation using RT-PCR-RFLP

Abstract

In the present study, 3 pooled proventricular homogenates were collected from 3 broiler flocks, of chicken 15 to 30 days old, from Monofia Governorate. The 3 flocks were suffered from low growth rate, poor feed conversion rate, uneven growth and increased mortalities. Necropsy of dead chickens revealed proventriculitis with increased proventriculus size. IBD viral antigen was detected in the pooled proventricular homogenate of each flock by AGPT using reference antibodies against IBDV and RT-PCR technique. No other viruses were detected; such as Reo virus, CAV, NDV, IBV and ALV-J. Further characterization of the IBDV isolates were conducted by RFLP assay on PCR products using MboI and BstOI restriction enzymes. Results demonstrate that the 3 IBDV isolates are identical in their RFLP pattern and related to the Del/E variant strain of IBDV.

Keywords

• ibd virus; genomic; rt-pcr-rflp; virus