The RcAGL61 gene affects petal number by regulating the transition between stamens and petals in Rosa chinensis

QI Yucen; YANG Shumin; SU Lin; YANG Yi; ZHANG Qixiang; PAN Huitang

doi:10.7606/j.issn.1000-4025.20230568

Xibei zhiwu xuebao (Jun 2024)

The RcAGL61 gene affects petal number by regulating the transition between stamens and petals in Rosa chinensis

QI Yucen,
YANG Shumin,
SU Lin,
YANG Yi,
ZHANG Qixiang,
PAN Huitang

Affiliations

QI Yucen: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
YANG Shumin: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
SU Lin: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
YANG Yi: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
ZHANG Qixiang: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
PAN Huitang: Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

DOI: https://doi.org/10.7606/j.issn.1000-4025.20230568
Journal volume & issue: Vol. 44, no. 6
pp. 904 – 911

Abstract

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Abstract [Objective] The study aims to elucidate the regulatory mechanism of the double petal trait in rose. We cloned an AG homologous gene RcAGL61, which was previously screened and related to flower development, and examined its function. [Methods] qRT-PCR was used to analyze the expression of RcAGL61 in flower buds of the double-petaled lines and single-petaled lines in Rosa chinensis ‘Zhaiye Tengben Yuejihua’ × R. chinensis‘Old Blush’ population. RcAGL61 was cloned from the double-petaled lines and single-petal lines, and bioinformatics analysis, subcellular localization, and VIGS experiments were carried out. [Results] (1) The expression of RcAGL61 was higher in the five developmental stages of the singlepetaled line than the double-petaled line, and the expression was higher in S4-S5 stages than in S1-S3 stages in the single-petaled line. (2) The coding sequence of RcAGL61 was the same in the double-petaled line and single-petaled line, with a length of 495 bp, which had a similarity of 30.75% with RcAG. RcAGL61 encoded 164 amino acids, which contained a conserved MADS-box, belonging to the MADS-box family. (3) RcAGL61 was localized in the nucleus. (4) When silenced by VIGS technique in R. chinensis ‘Old Blush’, the petalized stamens and petal numbers were increased, while the stamen numbers were decreased. The number of sepals and pistils had no significant change. [Conclusion] RcAGL61 is involved in regulating the transition between stamens and petal primordiums, and affects the number of petals in R. chinensis.

Published in Xibei zhiwu xuebao

ISSN: 1000-4025 (Print)
Publisher: Science Press
Country of publisher: China
LCC subjects: Science: Biology (General); Science: Botany
Website: http://xbzwxb.alljournal.net/xbzwxben/home

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