Genome-wide identification of TCP transcription factors and their potential roles in hydrolyzable tannin production in Quercus variabilis cupule

Yaochen Wang; Yaochen Wang; Yaochen Wang; Jinjin Li; Jinjin Li; Jinjin Li; Yixin Chen; Yixin Chen; Yixin Chen; Zhaowei Yu; Zhaowei Yu; Zhaowei Yu; Puyuan Liu; Puyuan Liu; Puyuan Liu; Guolei Li; Guolei Li; Guolei Li; Qinsong Yang; Qinsong Yang; Qinsong Yang

doi:10.3389/fpls.2024.1444081

Frontiers in Plant Science (Aug 2024)

Genome-wide identification of TCP transcription factors and their potential roles in hydrolyzable tannin production in Quercus variabilis cupule

Yaochen Wang,
Yaochen Wang,
Yaochen Wang,
Jinjin Li,
Jinjin Li,
Jinjin Li,
Yixin Chen,
Yixin Chen,
Yixin Chen,
Zhaowei Yu,
Zhaowei Yu,
Zhaowei Yu,
Puyuan Liu,
Puyuan Liu,
Puyuan Liu,
Guolei Li,
Guolei Li,
Guolei Li,
Qinsong Yang,
Qinsong Yang,
Qinsong Yang

Affiliations

Yaochen Wang: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Yaochen Wang: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Yaochen Wang: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China
Jinjin Li: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Jinjin Li: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Jinjin Li: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China
Yixin Chen: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Yixin Chen: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Yixin Chen: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China
Zhaowei Yu: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Zhaowei Yu: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Zhaowei Yu: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China
Puyuan Liu: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Puyuan Liu: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Puyuan Liu: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China
Guolei Li: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Guolei Li: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Guolei Li: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China
Qinsong Yang: Research Center of Deciduous Oaks, Beijing Forestry University, Beijing, China
Qinsong Yang: Deciduous Oak Improvement and Regeneration Innovation Team of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China
Qinsong Yang: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China

DOI: https://doi.org/10.3389/fpls.2024.1444081
Journal volume & issue: Vol. 15

Abstract

Read online

Hydrolyzable tannins (HTs) have garnered significant attention due to their proven beneficial effects in the clinical treatment of various diseases. The cupule of Chinese cork oak (Quercus variabilis Blume) has been used as raw material of traditional medicine for centuries for its high content of HTs. Previous studies have identified UGT84A13 as a key enzyme in the HT biosynthesis pathway in Q. variabilis, but the transcriptional regulation network of UGT84A13 remains obscure. Here, we performed a comprehensive genome-wide identification of the TCP transcription factors in Q. variabilis, elucidating their molecular evolution and gene structure. Gene expression analysis showed that TCP3 from the CIN subfamily and TCP6 from the PCF subfamily were co-expressed with UGT84A13 in cupule. Further functional characterization using dual-luciferase assays confirmed that TCP3, rather than TCP6, played a role in the transcriptional regulation of UGT84A13, thus promoting HT biosynthesis in the cupule of Q. variabilis. Our work identified TCP family members in Q. variabilis for the first time, and provided novel insights into the transcriptional regulatory network of UGT84A13 and HT biosynthesis in Q. variabilis, explaining the reason why the cupule enriches HTs that could be used for traditional medicine.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

About the journal

Abstract

Keywords