Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

Xiong Ding; Kun Yin; Ziyue Li; Rajesh V. Lalla; Enrique Ballesteros; Maroun M. Sfeir; Changchun Liu

doi:10.1038/s41467-020-18575-6

Nature Communications (Sep 2020)

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

Xiong Ding,
Kun Yin,
Ziyue Li,
Rajesh V. Lalla,
Enrique Ballesteros,
Maroun M. Sfeir,
Changchun Liu

Affiliations

Xiong Ding: Department of Biomedical Engineering, University of Connecticut Health Center
Kun Yin: Department of Biomedical Engineering, University of Connecticut Health Center
Ziyue Li: Department of Biomedical Engineering, University of Connecticut Health Center
Rajesh V. Lalla: Section of Oral Medicine, University of Connecticut Health Center
Enrique Ballesteros: Department of Pathology and Laboratory Medicine, University of Connecticut Health Center
Maroun M. Sfeir: Department of Pathology and Laboratory Medicine, University of Connecticut Health Center
Changchun Liu: Department of Biomedical Engineering, University of Connecticut Health Center

DOI: https://doi.org/10.1038/s41467-020-18575-6
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Rapid and early detection of SARS-CoV-2 will aid intervention to stop disease spread. Here the authors present a one-pot CRISPR-based rapid detection system with visual readout.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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