Терапевтический архив (Sep 2009)

The influence of coronary stenting with rapamycin-eluting stents on the number of circulating CD4+CD25high+regulatory T-cells

  • Tat'yana Igorevna Aref'eva,
  • Sergey Il'ich Provatorov,
  • Aleksandra Viktorovna Potekhina,
  • Vladimir Olegovich Sokolov,
  • Nadezhda Borisovna Kukhtina,
  • Anatoliy Nikolaevich Samko,
  • Tat'yana Leonidovna Krasnikova,
  • T I Arefieva,
  • S I Provatorov,
  • A V Potekhina,
  • V O Sokolov,
  • N B Kukhtina,
  • A N Samko,
  • T L Krasnikova

Journal volume & issue
Vol. 81, no. 9
pp. 33 – 37

Abstract

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Aim. To investigate the effects of coronary stenting with rapamycin-eluting stents on parameters of cell immunity. Methods. 26 patients (group 1) with stable coronary heart disease and angiographically proved coronary stenosis underwent stenting with rapamycin-eluting stents. The control group (group 2) consisted of 6 patients: 4 patients underwent diagnostic coronaroangiography, 1 patient got a bare metal stent and in 1 patient angioplasty was unsuccessful. Blood samples were obtained before and 1 month after the intervention. The quantity of activated (CD4+CD25low+) and regulatory (CD4+CD25high+) T cells was measured by direct immunofluorescence and flow cytometry. Plasma concentration of IL-10 was determined by ELISA. Results. In group 1 the percentages of CD4+CD25high+ regulatory T-cells increased significantly one month after stenting, while in group 2 no difference in regulatory T-cell levels before and after the intervention was observed. No changes in total number of leukocytes, relative levels of lymphocytes, CD4+ T-cells, activated CD4+CD25+low T-cells and IL-10 plasma concentration before and after the procedure were detected in both groups. Conclusion. Rapamycin-eluting stent implantation is associated with a significant increase of circulating CD4+CD25high+ regulatory T-cell level.

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