Integrated analysis of competitive endogenous ribose nucleic acids (ceRNAs)-related regulatory networks in invasive and non-invasive non-functioning pituitary adenomas (NFPAs)

Jiangtao Liu; Kaixuan Wang; Hongming Ji; Gangli Zhang; Shengli Chen; Shiyuan Zhang; Fake Lu; Changchen Hu; Changchen Hu

doi:10.3389/fsurg.2022.983958

Frontiers in Surgery (Sep 2022)

Integrated analysis of competitive endogenous ribose nucleic acids (ceRNAs)-related regulatory networks in invasive and non-invasive non-functioning pituitary adenomas (NFPAs)

Jiangtao Liu,
Kaixuan Wang,
Hongming Ji,
Gangli Zhang,
Shengli Chen,
Shiyuan Zhang,
Fake Lu,
Changchen Hu,
Changchen Hu

Affiliations

Jiangtao Liu: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Kaixuan Wang: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Hongming Ji: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Gangli Zhang: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Shengli Chen: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Shiyuan Zhang: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Fake Lu: Department of Biomedical Engineering, Binghamton University, State University of New York, Binghamton, United States
Changchen Hu: Department of Neurosurgery, Shanxi Provincial People’s Hospital, Shanxi Medical University, Taiyuan, China
Changchen Hu: Department of Neurosurgery, Shuozhou People’s Hospital, Shuozhou, China

DOI: https://doi.org/10.3389/fsurg.2022.983958
Journal volume & issue: Vol. 9

Abstract

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BackgroundThis study aims to identify the differentially expressed (DE) non-coding ribose nucleic acids (ncRNAs), messenger RNA (mRNA) expression profiles, and competitive endogenous RNA (ceRNA)-related regulatory networks in invasive and non-invasive nonfunctioning pituitary adenomas (NFPAs).MethodsA full-transcriptome sequencing of invasive and non-invasive NFPAs is carried out to evaluate the expression profiles of circular RNAs (circRNAs), long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNA expression profiles.ResultsThe screening criteria resulted in 118 DEcircRNAs (88 up-regulated and 30 down-regulated), 105 DElncRNAs (68 up-regulated and 37 down-regulated), 43 DEmiRNAs (22 up-regulated and 21 down-regulated), and 268 DEmRNAs (194 up-regulated and 74 down-regulated). Accordingly, a ceRNA regulatory network related to invasive NFPA is constructed. Further, the Gene Ontology and Kyoto Encylopedia of Genes and Genomes analyses showed that circRNAs and lncRNAs in the network are related to chromatin remodeling, participating in the Janus kinase/signal transducer and activator of transcription (JAK-STAT) and calcium signaling pathways. Hsa-miR-1248 showed exceptional connectivity in the ceRNA regulatory network, which could be closely related to the invasiveness of NFPAs.ConclusionsTogether, these findings clarified the regulatory mechanisms of invasive and non-invasive NFPAs, providing innovative research avenues and therapeutic targets for invasive NFPAs.

Published in Frontiers in Surgery

ISSN: 2296-875X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Surgery
Website: https://www.frontiersin.org/journals/surgery

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