Concentration Dependent Effect of Quaternary Amines on the Adhesion of U251-MG Cells

Abstract

Cationic gels have seen increasing interest in recent years for 2D cell cultivation since they may represent an alternative to the well-known RGD-peptide motif functionalized gels. However, few hydrogel systems with adjustable cationic strength have been fabricated and investigated so far. In this work, eight gels with defined concentrations of cationic groups, two of which also contained the RGD peptide, were prepared from three well-defined, soluble precursor copolymers with thiol-functionalities and PEGDA3500 as a crosslinker via thiol-ene chemistry. Live/dead stainings of U-251-MG cells on the hydrogels with different concentrations of the cationic motif were made after 3 days and 7 days of cultivation. The results show a high dependence of the number of adhesive cells and their morphology, cluster versus spread cells, on the concentration of cationic groups in the gel. This effect was more pronounced when the gels were not further dialyzed before usage. In addition, a synergistic effect of the two motifs, cationic group and RGD peptide, could be demonstrated, which together induce stronger cell adhesion than either motif alone.

Keywords

• cationic hydrogels
• U251-MG cells
• cell adhesion
• live/dead assay