De novo transcriptome assembly of textile hemp from datasets on hypocotyls and adult plants
Marc Behr,
Stanley Lutts,
Jean-Francois Hausman,
Kjell Sergeant,
Sylvain Legay,
Gea Guerriero
Affiliations
Marc Behr
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, Z.A.E. Robert Steichen, L-4940, Hautcharage, Luxembourg; Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute - Agronomy (ELI-A), Université catholique de Louvain (UCLouvain), 1348 Louvain-la-Neuve, Belgium
Stanley Lutts
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute - Agronomy (ELI-A), Université catholique de Louvain (UCLouvain), 1348 Louvain-la-Neuve, Belgium
Jean-Francois Hausman
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, Z.A.E. Robert Steichen, L-4940, Hautcharage, Luxembourg
Kjell Sergeant
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, Z.A.E. Robert Steichen, L-4940, Hautcharage, Luxembourg
Sylvain Legay
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, Z.A.E. Robert Steichen, L-4940, Hautcharage, Luxembourg
Gea Guerriero
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5, rue Bommel, Z.A.E. Robert Steichen, L-4940, Hautcharage, Luxembourg; Corresponding author. Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, 4362 Esch/Alzette, Luxembourg.
We here provide an updated de novo transcriptome of the hemp textile variety Santhica 27. The assembly was performed by merging the reads obtained previously on a time-series relative to the hypocotyl development and on bast fibers isolated from internodes of adult plants at different heights with those obtained from a newly performed transcriptome study on the hypocotyl in response to jasmonic acid treatment. More specifically, hypocotyls aged 15 days were treated with jasmonic acid and collected 3 and 5 days after the application of the plant growth regulator. RNA-Seq was then performed on the treated hypocotyls. The transcriptome reported here will be a useful resource for those scientists engaged in the study of bast fiber development, as well as cell wall biosynthesis in textile hemp. The transcriptome is also useful for molecular studies relative to the synthesis of secondary metabolites, such as phenolic compounds (e.g. flavonoids) and lignans/lignanamides. Keywords: Hemp, Cell wall, Transcriptome, Hypocotyl, Bast fiber