Biological Activity of Cyclic Peptide Extracted from <i>Sphaeranthus amaranthoides</i> Using De Novo Sequencing Strategy by Mass Spectrometry for Cancer
Swarnalatha Yanamadala,
Sivakumar Shanthirappan,
Sidhika Kannan,
Narendran Chiterasu,
Kumaran Subramanian,
Lamya Ahmed Al-Keridis,
Tarun Kumar Upadhyay,
Nawaf Alshammari,
Mohd Saeed,
Guru Prasad Srinivasan,
Rohini Karunakaran
Affiliations
Swarnalatha Yanamadala
Department of Biotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, India
Sivakumar Shanthirappan
Department of Gunapadam, National Institute of Siddha, Chennai 600047, India
Sidhika Kannan
Centre for Nanoscience and Nanotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, India
Narendran Chiterasu
Centre for Nanoscience and Nanotechnology, Sathyabama Institute of Science and Technology, Chennai 600119, India
Kumaran Subramanian
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai 600119, India
Lamya Ahmed Al-Keridis
Department Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh P.O. Box 84428, Saudi Arabia
Tarun Kumar Upadhyay
Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, India
Nawaf Alshammari
Department of Biology, College of Science University of Hail, Hail P.O. Box 2440, Saudi Arabia
Mohd Saeed
Department of Biology, College of Science University of Hail, Hail P.O. Box 2440, Saudi Arabia
Guru Prasad Srinivasan
Centre for Research, Bharath Institute for Higher Education and Research, Chennai 600073, India
Rohini Karunakaran
Unit of Biochemistry, Faculty of Medicine, AIMST University, Bedong 08100, Malaysia
Though there are several advancements and developments in cancer therapy, the treatment remains challenging. In recent years, the antimicrobial peptides (AMPs) from traditional herbs are focused for identifying and developing potential anticancer molecules. In this study, AMPs are identified from Sphaeranthus amaranthoides, a natural medicinal herb widely used as a crucial immune stimulant in Indian medicine. A total of 86 peptide traces were identified using liquid-chromatography–electrospray-ionisation mass spectrometry (LC-ESI-MS). Among them, three peptides were sequenced using the manual de novo sequencing technique. The in-silico prediction revealed that SA923 is a cyclic peptide with C-N terminal interaction of the carbon atom of ASP7 with the nitrogen atom of GLU1 (1ELVFYRD7). Thus, SA923 is presented under the orbitides class of peptides, which lack the disulfide bonds for cyclization. In addition, SA923, steered with the physicochemical properties and support vector machine (SVM) algorithm mentioned for the segment, has the highest in silico anticancer potential. Further, the in vitro cytotoxicity assay revealed the peptide has anti-proliferative activity, and toxicity studies were demonstrated in Danio rerio (zebrafish) embryos.
