Frontiers in Immunology (Mar 2024)

Co-formulation of the rF1V plague vaccine with depot-formulated cytokines enhances immunogenicity and efficacy to elicit protective responses against aerosol challenge in mice

  • Darrell R. Galloway,
  • Jiahui Li,
  • Nguyen X. Nguyen,
  • Frank W. Falkenberg,
  • Frank W. Falkenberg,
  • Lisa Henning,
  • Robert Krile,
  • Ying-Liang Chou,
  • James N. Herron,
  • J. Scott Hale,
  • E. Diane Williamson

DOI
https://doi.org/10.3389/fimmu.2024.1277526
Journal volume & issue
Vol. 15

Abstract

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This study evaluated a depot-formulated cytokine-based adjuvant to improve the efficacy of the recombinant F1V (rF1V) plague vaccine and examined the protective response following aerosol challenge in a murine model. The results of this study showed that co-formulation of the Alhydrogel-adsorbed rF1V plague fusion vaccine with the depot-formulated cytokines recombinant human interleukin 2 (rhuIL-2) and/or recombinant murine granulocyte macrophage colony-stimulating factor (rmGM-CSF) significantly enhances immunogenicity and significant protection at lower antigen doses against a lethal aerosol challenge. These results provide additional support for the co-application of the depot-formulated IL-2 and/or GM-CSF cytokines to enhance vaccine efficacy.

Keywords