HCC1, a Polygalacturonase, Regulates Chlorophyll Degradation via the Ethylene Synthesis Pathway

Yongxiang Liao; Bing Xiang; Zhenzhen Xue; Asif Ali; Yong Li; Mengyuan Li; Aiji Wei; Jialu Xin; Daiming Guo; Yingxiu Liao; Yunfeng Tian; Zhixue Zhao; Peizhou Xu; Hongyu Zhang; Xiaoqiong Chen; Yutong Liu; Hao Zhou; Duo Xia; Kangxi Du; Xianjun Wu

doi:10.1186/s12284-023-00675-8

Rice (Dec 2023)

HCC1, a Polygalacturonase, Regulates Chlorophyll Degradation via the Ethylene Synthesis Pathway

Yongxiang Liao,
Bing Xiang,
Zhenzhen Xue,
Asif Ali,
Yong Li,
Mengyuan Li,
Aiji Wei,
Jialu Xin,
Daiming Guo,
Yingxiu Liao,
Yunfeng Tian,
Zhixue Zhao,
Peizhou Xu,
Hongyu Zhang,
Xiaoqiong Chen,
Yutong Liu,
Hao Zhou,
Duo Xia,
Kangxi Du,
Xianjun Wu

Affiliations

Yongxiang Liao: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Bing Xiang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Zhenzhen Xue: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Asif Ali: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Yong Li: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Mengyuan Li: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Aiji Wei: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Jialu Xin: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Daiming Guo: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Yingxiu Liao: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Yunfeng Tian: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Zhixue Zhao: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Peizhou Xu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Hongyu Zhang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Xiaoqiong Chen: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Yutong Liu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Hao Zhou: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Duo Xia: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Kangxi Du: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University
Xianjun Wu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University

DOI: https://doi.org/10.1186/s12284-023-00675-8
Journal volume & issue: Vol. 16, no. 1
pp. 1 – 16

Abstract

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Abstract Chlorophyll degradation is an important physiological process and is essential for plant growth and development. However, how chlorophyll degradation is controlled at the cellular and molecular level remains largely elusive. Pectin is a main component of the primary cell wall, and polygalacturonases (PGs) is a group of pectin-hydrolases that cleaves the pectin backbone and release oligogalacturonide. Whether and how PGs affect chlorophyll degradation metabolism and its association with ethylene (ETH) have not been reported before. Here, we report a novel function of PG in a mutant ‘high chlorophyll content1’ hcc1, which displayed a decrease in growth and yield. Our morphological, biochemical and genetic analyses of hcc1, knockout lines and complementation lines confirm the function of HCC1 in chlorophyll degradation. In hcc1, the PG activity, ETH content and D-galacturonic acid (D-GA) was significantly decreased and showed an increase in the thickness of the cell wall. Exogenous application of ETH and D-GA can increase ETH content and induce the expression of HCC1, which further can successfully induce the chlorophyll degradation in hcc1. Together, our data demonstrated a novel function of HCC1 in chlorophyll degradation via the ETH pathway.

Published in Rice

ISSN: 1939-8425 (Print); 1939-8433 (Online)
Publisher: SpringerOpen
Country of publisher: United Kingdom
LCC subjects: Agriculture: Plant culture
Website: https://thericejournal.springeropen.com/

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