The Effect of Interval Training With Resveratrol on Some Apoptotic Indices of the Heart Tissue of Rats With Diabetes Caused by Streptozotocin

Abstract

Background: Today, diabetes is considered one of the main causes of death so that the aim of this study was to investigate the effect of interval training with resveratrol on some apoptotic indices of the hepatocyte tissue in diabetic male rats. Materials and Methods: In this experimental trial, 42 male rats were randomly divided into 6 groups, including diabetic saline, diabetic, healthy, supplement, interval exercise, and resveratrol supplementation+interval exercise (7 mice in each group). The program of interval training groups and resveratrol supplementation+interval training on the treadmill included 3 sessions per week, and each session was 10 sets of 1-minute activity that started with 14 meters per minute and reached 28 meters per minute in the eighth week. BCL2, BAX, and CASPASE-3 levels were determined by enzyme-linked immunosorbent assay kits (Zelbio, Germany, Catalog: RK03522, RK03549). Results: CASPASE and BAX -3 levels were higher in diabetic-saline and diabetic groups compared to the other groups (P=0.001). Bcl-2 levels were lower in diabetic-saline and diabetic groups in comparison to other groups (P=0.001). The mean BAX/BCL2 ratio was higher in diabetic-saline and diabetic groups (P=0.001). Interval exercise and resveratrol administration alone or especially resveratrol intervention combined with interval exercise caused a significant decrease in mean CASPASE-3 and BAX concentrations (P=0.001), BAX/BCL2 ratio (P=0.001), while a significant increase in the BCL-2 concentration (P=0.001) in the myocyte tissue. Conclusion: The results demonstrated that exercise could increase BAX and BCL-2. This improvement was greater when combined with resveratrol.

Keywords

• interval training
• resveratrol
• apoptosis
• diabetes