An optimized workflow to measure bacterial predation in microplates

Abstract

Summary: The predatory bacterium Bdellovibrio bacteriovorus invades and proliferates inside other bacteria by non-binary division. Here we describe a fluorescence-based technique for the immediate evaluation of predator density independently of plaque formation, an optimized setup to monitor predation in microplates, and the CuRveR package to quantify both prey killing and predator proliferation dynamics. This protocol allows to assess the impact of mutations or chemicals on predation. CuRveR also constitutes a user-friendly tool to analyze growth or decay data unrelated to predation.For complete details on the use and execution of this profile, please refer to Kaljević et al., 2021.

Keywords

• Cell Biology
• Cell-based Assays
• Microbiology
• Molecular/Chemical Probes