International Journal of Hematology-Oncology and Stem Cell Research (Dec 2016)
The Relationship between STR-PCR Chimerism Analysis and Chronic GvHD following Hematopoietic Stem Cell Transplantation
Abstract
Background: The study attempts to assess the relationship between chimerism analysis using polymerase chain reaction of short tandem repeat (STR) and the incidence of chronic graft versus host disease (GvHD) as well as survival. Methods: The retrospective cohort included all patients who received allo-HSCT between 2005 to 2013. Data collected by day +100 were reviewed in terms of the incidence of chronic GvHD and survival. Chimerism was evaluated for whole blood, T-cell and PMN cells on days 15, 30 and 60, respectively using polymerase chain reaction of short tandem repeat (STR). Results: Forty (69%) patients developed chronic GvHD, 11 (19%) relapsed and 22 (39.7%) died during the study. There was a significant relationship between chronic GvHD and chimerism analysis including whole blood on day 60 (P=0.001), PMN on day 60 (p= 0.05) and T-cell on days 15 (p=0.028), 30(p=0.01) and 60 (p=0.004). Patients with chronic GvHD showed a long-term survival as compared with those without chronic GvHD (P=0.0013). Conclusion: Conducting continuous analysis of chimerism provides an opportunity to initiate immediate measures to prevent complications.
