中链甘油三酯中甘油二酯的分离与结构鉴定Isolation and identification of diglycerides from medium-chain triglycerides

Abstract

旨在阐明中链甘油三酯产品中的主要副产物甘油二酯类杂质的化学成分，以中链甘油三酯为原料，通过硅胶柱层析和半制备高效液相色谱法分离其中的甘油二酯，并通过核磁谱、质谱、红外光谱、紫外光谱和气相色谱对甘油二酯的结构进行鉴定。结果表明：从中链甘油三酯中分离出6种甘油二酯（DG-1~DG-6），结构依次被鉴定为1，3-二辛酸甘油二酯、1，2-二辛酸甘油二酯、1，3-辛酸癸酸甘油二酯、1，2-辛酸癸酸甘油二酯、1,3-二癸酸甘油二酯、1,2-二癸酸甘油二酯。了解中链甘油三酯中甘油二酯的结构有助于中链甘油三酯的质量控制和临床应用。To clarify the chemical composition of diglycerides impurities, a major by-product in medium-chain triglycerides products, diglycerides were isolated from medium-chain triglycerides through silica gel column and semipreparative high performance liquid chromatography, and the structures of diglycerides were identified. The results showed that six diglycerides (DG-1-DG-6) were isolated from medium-chain triglycerides, and the structures of DG-1-DG-6 were identified as 1,3-dicaprylin glycerate, 1,2-dicaprylin glycerate, 1,3-caprylin, caprin glycerate, 1,2-caprylin, caprin glycerate, 1,3-dicaprin glycerate, 1,2-dicaprin glycerate by nuclear magnetic spectrum, mass spectrum, IR spectrum, ultraviolet spectrum, and gas chromatography. Knowing the structure of diglycerides in medium-chain triglycerides is conducive to the quality control and clinical application of medium-chain triglycerides.

Keywords

• 中链甘油三酯；甘油二酯；分离；鉴定
• medium-chain triglycerides; diglycerides; isolation; identification