Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Abstract

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.

Keywords

• COVID-19
• SARS-CoV-2
• rRT-PCR
• molecular detection
• respiratory infections
• severe acute respiratory syndrome coronavirus 2