Eclética Química (Apr 2018)
Validação do método de extração e quantificação de 7-hidróxi- 4’,6-dimetóxi-isoflavona em culturas de células em suspensão e calos de Dipteryx odorata
Abstract
The present work consists of the development and validation of HPLC analytical methodology for the evaluation of 7-hydroxy-4’, 6-dimethoxyisoflavone in cells suspension and callus culture of Dipteryx odorata. The validation parameters: analytical curve, linearity, precision, recovery, detection limit and quantification limit were determined and they demonstrated that the analytical procedures for the detection and quantification for this isoflavone follow the requeriments of regulatory RE899/03- National Health Surveillance Agency (ANVISA) and could be applied to cells culture quality control of this vegetal species.