Taiwanese Journal of Obstetrics & Gynecology (Jun 2009)

Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction

  • Chi-Feng Wei,
  • Bo-Ching She,
  • Hung-Shuo Liang,
  • Qing-Dong Ling,
  • Chen-Yuan Tsai,
  • Chih-Wei Yen,
  • Hsueh-Yin Wu,
  • Ming-Song Tsai

Journal volume & issue
Vol. 48, no. 2
pp. 116 – 119


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Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation. Materials and Methods: From 2006 to 2007, we recruited 150 pregnant women who received routine GBS culture screening at 35 weeks' gestation with negative results, and who had repeat cultures and real-time polymerase chain reaction (RT-PCR) tests for GBS after 39 weeks' gestation. Results: Two percent of pregnant women with GBS-negative results by culture screening at 35 weeks' gestation were GBS-positive at 39 weeks' gestation. Conclusion: It is necessary to perform a GBS test 4 weeks after an initial negative GBS culture at 35–37 weeks of gestation. RT-PCR provides a simple and rapid alternative method for detecting rectovaginal GBS colonization at the time of labor.