Shiyan dongwu yu bijiao yixue (Aug 2022)

A Preliminary Method for Continuous Drainage of Mesenteric Lymph Fluid in Rats

  • ZHANG Xiaorui,
  • CAO Jing,
  • WU Qianqian,
  • KANG Kang,
  • CHEN Guoyuan,
  • WU Baojin

DOI
https://doi.org/10.12300/j.issn.1674-5817.2022.024
Journal volume & issue
Vol. 42, no. 4
pp. 267 – 274

Abstract

Read online

ObjectiveTo establish a novel sustained collection method for mesenteric lymph fluid by means of assisted reflux from the mesenteric-jugular lymphatic duct in rats.MethodsSixteen male Sprague-Dawley (SD) rats were randomly divided into two groups. The control group underwent duodenal and intestinal lymphatic duct cannulation, after which intestinal lymph fluid was collected. The experimental group underwent jugular vein and intestinal lymphatic duct cannulation to establish intestinal-jugular lymphatic duct assisted reflux. The intestinal lymph fluid was collected on the 7th day after the operation using an awake mobility device. The flow rate of intestinal lymph fluid was recorded, and its cellular components and some biochemical indicators were detected.ResultsMesenteric-jugular lymphatic duct vein cannulation assisted reflux was successfully established in rats, and the rat models could be maintained for more than seven days. The intestinal lymph flow rate was （2.01±0.12） mL/h in the experimental group, which was higher than that of the control group [(0.92±0.09) mL/h, P＜0.01]. The number of lymphocytes (LYM#) and percentage of lymphocytes (LYM/%) in the experimental group were higher than those in the control group (P＜0.01). The percentage of neutrophils (NEUT/%) and percentage of monocytes (MONO/%) were lower than those in the control group (P＜0.01, P＜0.05, respectively). The concentrations of K+, Na+, CO2, and urea in the lymph fluid of the experimental group were higher than those of the control group (P＜0.01). However, the concentrations of triacylglycerol (TG) and P3+ were lower than those of the control group (P＜0.01).ConclusionThis novel method can achieve real-time and long-term collection of mesenteric lymph fluid in rats under the condition of being awake, unrestricted in diet and in normal state, avoiding the influence of surgical stress, general anesthesia or animal restraint on the experimental results.

Keywords