Identification of quantitative trait loci (QTLs) regulating leaf SPAD value and trichome density in mungbean (Vigna radiata L.) using genotyping-by-sequencing (GBS) approach
Nikki Kumari,
Gyan Prakash Mishra,
Harsh Kumar Dikshit,
Soma Gupta,
Anirban Roy,
Subodh Kumar Sinha,
Dwijesh C. Mishra,
Shouvik Das,
Ranjeet R. Kumar,
Ramakrishnan Madhavan Nair,
Muraleedhar Aski
Affiliations
Nikki Kumari
Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India
Gyan Prakash Mishra
Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India
Harsh Kumar Dikshit
Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India
Soma Gupta
Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India
Anirban Roy
Plant Pathology, Indian Agricultural Research Institute, New Delhi, Delhi, India
Subodh Kumar Sinha
Biotechnology, National Institute of Plant Biotechnology, New Delhi, Delhi, India
Dwijesh C. Mishra
Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute, New Delhi, Delhi, India
Shouvik Das
Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India
Ranjeet R. Kumar
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, Delhi, India
Ramakrishnan Madhavan Nair
ICRISAT, World Vegetable Center, South Asia, ICRISAT Campus, Patancheru, Hyderabad, India
Muraleedhar Aski
Genetics, Indian Agricultural Research Institute, New Delhi, Delhi, India
Quantitative trait loci (QTL) mapping is used for the precise localization of genomic regions regulating various traits in plants. Two major QTLs regulating Soil Plant Analysis Development (SPAD) value (qSPAD-7-1) and trichome density (qTric-7-2) in mungbean were identified using recombinant inbred line (RIL) populations (PMR-1×Pusa Baisakhi) on chromosome 7. Functional analysis of QTL region identified 35 candidate genes for SPAD value (16 No) and trichome (19 No) traits. The candidate genes regulating trichome density on the dorsal leaf surface of the mungbean include VRADI07G24840, VRADI07G17780, and VRADI07G15650, which encodes for ZFP6, TFs bHLH DNA-binding superfamily protein, and MYB102, respectively. Also, candidate genes having vital roles in chlorophyll biosynthesis are VRADIO7G29860, VRADIO7G29450, and VRADIO7G28520, which encodes for s-adenosyl-L-methionine, FTSHI1 protein, and CRS2-associated factor, respectively. The findings unfolded the opportunity for the development of customized genotypes having high SPAD value and high trichome density having a possible role in yield and mungbean yellow vein mosaic India virus (MYMIV) resistance in mungbean.
