Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications

Bioengineering. 2018;5(2):43 DOI 10.3390/bioengineering5020043

 

Journal Homepage

Journal Title: Bioengineering

ISSN: 2306-5354 (Online)

Publisher: MDPI AG

LCC Subject Category: Technology | Science: Biology (General)

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML, XML

 

AUTHORS

Katharina Schimek (TissUse GmbH, D-13347 Berlin, Germany)
Hao-Hsiang Hsu (Institute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, Germany)
Moritz Boehme (Institute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, Germany)
Jacob Jan Kornet (Institute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, Germany)
Uwe Marx (TissUse GmbH, D-13347 Berlin, Germany)
Roland Lauster (Institute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, D-13355 Berlin, Germany)
Ralf Pörtner (Institute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, Germany)
Gerd Lindner (Institute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, D-13355 Berlin, Germany)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 6 weeks

 

Abstract | Full Text

The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays.